Together, these considerations suggest that NR0B1 is a DNA-binding TF, and NR0B2 does not bind DNA, and is rather a co-factor.đ6709599 TF PPI Other Medium In mammals: "DAX1 (NR0B1), and SHP (NR0B2) are atypical nuclear receptor superfamily members that function primarily as corepressors through heterodimeric interactions with other nuclear receptors." HMMER hmmpfam analysis of human NR0B2 with the Pfam database shows PF00104.17 (Ligand-binding domain of nuclear hormone) as the top hit with a E-value of 6.9e-32, and PF03681.4 (Uncharacterised protein family) as a distant second with and E-value of 0.0022. Indeed, the canonical length of human NR0B1 is 470 AA, while the canonical length of human NR0B2 is 257 AA, consistent with the presence an extra domain (e.g., DNA binding) in NR0B1. The NCBI GEnRIF for human NR0B1 states "This gene encodes a protein that contains a DNA-binding domain", while the entry for human NR0B2 states "The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain". The SAP (after SAF-A/B, Acinus and PIAS) motif is a putative DNA binding domain found in diverse nuclear proteins. HMMER hmmpfam analysis of human NR0B1 with the Pfam database shows PF00104.17 (Ligand-binding domain of nuclear hormone) as the top hit with a E-value of 4.3e-28, and PF02037.14 (SAP domain) as a distant second with and E-value of 0.00041. Gene IDĝescriptionĝBD Classification (Protein Group/Protein Family) Reviewer Judgement Taxa Reviewer Commments PubMed IDğunction SpeciesĞvidence Strength PubMed Commentsġ1614 nuclear receptor subfamily 0, group B, member 1 Jared Roach TF GeneĝNA-Binding: sequence-specific NR0B1 and NR0B2 when analyzed by NCBI's "Conserved Domain" tool show only the HOLI domain (Ligand binding domain of hormone receptors ).
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